THE NUCLEIC ACIDS OF BASAL BODIES ISOLATED FROM TETRAHYMENA PYRIFORMIS
نویسندگان
چکیده
منابع مشابه
The Nucleic Acids of Basal Bodies Isolated from Tetrahymena Pyriformis
Pellicular fragments were isolated from ethanol-fixed cells of the holotrichous ciliate Tetrahymena pyriformis by the action of digitonin. The isolated pellicles were further fragmented and the basal bodies of the cilia isolated from them by three methods. The preparations, examined in the electron microscope as embedded sections or negatively stained samples, consisted mainly of somewhat defor...
متن کاملThe Isolation of Ciliary Basal Bodies (kinetosomes) from Tetrahymena Pyriformis
S e a m a n (7, 8) has devised a m e t h o d for the isolation of ciliary basal bodies (kinetosomes) from Tetrahymena pyriformis a n d has presented d a t a on the c h e m i c a l properties of his fraction. However, only light m i c r o s c o p y was e m p l o y e d as a m e a n s of identification of the kinetosomes. I n the experi-m e n t s described here the m a t e r i a l o b t a i n e d ...
متن کاملTransitory Heterosis in Numbers of Basal Bodies in TETRAHYMENA PYRIFORMIS.
The numbers of kinetosomes in ciliary rows on the ventral surface of cells of Tetrahymena pyriformis, syngen 1, are greater by about 10% in young heterozygotes established by crossing two inbred strains than in either homozygote. The difference, apparent at 20 fissions post-fertilization, is essentially gone by 100 fissions. This observation is consistent with a generalized "allelic exclusion" ...
متن کاملStructural analysis of basal bodies of the isolated oral apparatus of Tetrahymena pyriformis.
The oral apparatus of Tetraliymena pyriformis was isolated using a non-ionic detergent to disrupt the cell membrane. The mouth consists largely of basal bodies and microfilaments. Each basal body is attached to the mouth by a basal plate which is integrated into the meshwork of microfilaments that confers upon the oral apparatus its structural integrity. Each basal body is composed of 9 triplet...
متن کاملPartial Purification and Phosphotungstate Solubilization of Basal Bodies and Kinetodesmal Fibers from Tetrahymena Pyriformis
Previously devised methods for the isolation of basal bodies from ciliate protozoans were found to be inadequate for chemical analysis. We have modified and expanded these procedures and developed a method which gives preparations containing mainly basal bodies and kinetodesmal fibers. This procedure involved fixation of cells in 30% ETOH followed by digitonin or Triton X-100 solubilization and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1965
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.25.2.217